Human βTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit

Specifications

Catalog number

E-EL-H1007-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ΒTG; PBP; CXCL7; NAP2

UniProt

P02775

Cross Reactivity

This kit recognizes Human βTG/PBP/CXCL7/NAP2 in samples.No significant cross-reactivity or interference between Human βTG/PBP/CXCL7/NAP2 and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cardiovascular; Immunology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human βTG/PBP/CXCL7/NAP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human βTG/PBP/CXCL7/NAP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human βTG/PBP/CXCL7/NAP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human βTG/PBP/CXCL7/NAP2. You can calculate the concentration of Human βTG/PBP/CXCL7/NAP2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Plasma

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797026/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human βTG/PBP/CXCL7/NAP2 concentrations in plasma.