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Product details

Human VIM (Vimentin) ELISA Kit

Catalog number E-EL-H1094-01

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Price for E-EL-H1094-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-H1094-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
VIM
UniProt
P08670
Cross Reactivity
This kit recognizes Human VIM in samples. No significant cross-reactivity or interference between Human VIM and analogues was observed
Target
VIM; FLJ36605
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Developmental biology; Neuroscience; Signal transduction; Stem cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VIM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VIM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VIM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VIM. You can calculate the concentration of Human VIM in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.69 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.mdpi.com/984018
Citation 02
https://www.mdpi.com/1494566
Citation 03
https://www.mdpi.com/2075-4418/12/8/1939
Citation 04
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238117
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human VIM concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human VIM (Vimentin) ELISA Kit?

The catalog number for Human VIM (Vimentin) ELISA Kit is E-EL-H1094.

What sizes are available for Human VIM (Vimentin) ELISA Kit?

Human VIM (Vimentin) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human VIM (Vimentin) ELISA Kit come in?

Human VIM (Vimentin) ELISA Kit is available in 3 different pack sizes.

Is Human VIM (Vimentin) ELISA Kit in stock?

Human VIM (Vimentin) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human VIM (Vimentin) ELISA Kit?

Human VIM (Vimentin) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human VIM (Vimentin) ELISA Kit?

Estimated delivery time for Human VIM (Vimentin) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human VIM (Vimentin) ELISA Kit?

The safety data sheet for Human VIM (Vimentin) ELISA Kit is available here: https://789.bio/eb/z1CuD4.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

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