Human SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit

Specifications

Catalog number

E-EL-H1113-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

SOD1

UniProt

P00441

Cross Reactivity

This kit recognizes Human SOD1 in samples.No significant cross-reactivity or interference between Human SOD1 and analogues was observed

Target

ALS; ALS1; IPOA; Superoxide dismutase [Cu-Zn]

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human SOD1. During the reaction, Human SOD1 in the sample or standard competes with a fixed amount of Human SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/1007476

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332221004662

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/ina.13093

Citation 04

https://www.mdpi.com/2072-6643/13/5/1480

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086968/

Citation 06

https://www.sciencedirect.com/science/article/pii/S0169131720300612

Citation 07

https://www.mdpi.com/2072-6651/13/9/639

Citation 08

https://www.sciencedirect.com/science/article/pii/S0147651318302975

Citation 09

https://journals.sagepub.com/doi/abs/10.1177/15593258221116741

Citation 10

https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/iub.1321

Citation 11

https://journals.sagepub.com/doi/abs/10.1177/2050312121991246

Citation 12

https://www.sciencedirect.com/science/article/pii/S0019483217304078

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human SOD1 concentrations in serum, plasma and other biological fluids.