Human NGF (Nerve Growth Factor) ELISA Kit

Specifications

Catalog number

E-EL-H1205-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

NGF

UniProt

P01138

Cross Reactivity

This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed

Target

Beta-NGF; HSAN5; NGFB; NGF-B

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NGF. You can calculate the concentration of Human NGF in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1550413122004909

Citation 02

https://www.karger.com/Article/Abstract/490139

Citation 03

https://www.sciencedirect.com/science/article/pii/S1756464622002900

Citation 04

https://www.mdpi.com/2072-6643/12/5/1522

Citation 05

https://www.sciencedirect.com/science/article/pii/S0014488616303466

Citation 06

https://www.sciencedirect.com/science/article/pii/S0924977X22001286

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8506992/

Citation 08

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0731-y

Citation 09

https://www.nature.com/articles/s41598-018-34527-z

Citation 10

https://link.springer.com/article/10.1007/s00449-023-02847-8

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1002/nau.24487

Citation 12

https://link.springer.com/article/10.1007/s11332-022-00992-3

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human NGF concentrations in serum, plasma and other biological fluids.