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Product details

Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit

Catalog number E-EL-H1454-01

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Price for E-EL-H1454-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-H1454-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
TIMP-3
UniProt
P35625
Cross Reactivity
This kit recognizes Human TIMP-3 in samples. No significant cross-reactivity or interference between Human TIMP-3 and analogues was observed
Target
TIMP3; SFD; HSMRK222; K222; K222TA2
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cell Biology; Cardiovascular; Neuroscience; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TIMP-3. You can calculate the concentration of Human TIMP-3 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
62.5-4000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
37.5 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S075333222300077X
Citation 02
https://www.mdpi.com/1400396
Citation 03
https://www.mdpi.com/2077-0383/10/16/3683
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human TIMP-3 concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit?

The catalog number for Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit is E-EL-H1454.

What sizes are available for Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit?

Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit come in?

Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit is available in 3 different pack sizes.

Is Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit in stock?

Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit?

Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit?

Estimated delivery time for Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit?

The safety data sheet for Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit is available here: https://789.bio/eb/ffrDSS.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

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