Human SOST (Sclerostin) ELISA Kit

Specifications

Catalog number

E-EL-H1544-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

SOST

UniProt

Q9BQB4

Cross Reactivity

This kit recognizes Human SOST in samples. No significant cross-reactivity or interference between Human SOST and analogues was observed

Target

CDD; VBCH

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SOST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SOST. You can calculate the concentration of Human SOST in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://aap.onlinelibrary.wiley.com/doi/abs/10.1002/JPER.21-0494

Citation 02

https://www.sciencedirect.com/science/article/pii/S1043466618304095

Citation 03

https://link.springer.com/article/10.1007/s00296-019-04298-5

Citation 04

https://www.mdpi.com/1648-9144/56/9/435

Citation 05

https://www.sciencedirect.com/science/article/pii/S0211699520300540

Citation 06

https://www.tandfonline.com/doi/abs/10.1080/14767058.2018.1514383

Citation 07

https://www.degruyter.com/document/doi/10.1515/jpem-2020-0140/html

Citation 08

https://link.springer.com/article/10.1007/s11255-018-1935-5

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8525806/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human SOST concentrations in serum, plasma and other biological fluids.