Human SIRT1 (Sirtuin 1) ELISA Kit

Specifications

Catalog number

E-EL-H1546-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

SIRT1

UniProt

Q96EB6

Cross Reactivity

This kit recognizes Human SIRT1 in samples. No significant cross-reactivity or interference between Human SIRT1 and analogues was observed

Target

SIR2L1; NAD-dependent deacetylase sirtuin-1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Metabolism; Microbiology; Tags and Cell Markers

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SIRT1. You can calculate the concentration of Human SIRT1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8908926/

Citation 02

https://www.sciencedirect.com/science/article/pii/S2212429221003333

Citation 03

https://www.sciencedirect.com/science/article/pii/S0378874122001489

Citation 04

https://www.hindawi.com/journals/omcl/2018/2731289/abs/

Citation 05

https://www.hindawi.com/journals/omcl/2018/9391261/abs/

Citation 06

https://dmsjournal.biomedcentral.com/articles/10.1186/s13098-021-00736-2

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human SIRT1 concentrations in serum, plasma and other biological fluids.