Human HMGB-1 (High Mobility Group Protein B1) ELISA Kit

Specifications

Catalog number

E-EL-H1554-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

HMGB-1

UniProt

P09429

Cross Reactivity

This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed

Target

HMGB1; HMG1; HMG3; SBP-1; Amphoterin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Epigenetics and Nuclear Signaling

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2211383522005214

Citation 02

https://link.springer.com/article/10.1186/s12951-021-01017-w

Citation 03

https://academic.oup.com/schizophreniabulletin/article-abstract/47/2/530/5911245

Citation 04

https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02535-6

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/liv.15457

Citation 06

https://www.sciencedirect.com/science/article/pii/S0142961218305209

Citation 07

https://www.frontiersin.org/articles/10.3389/fimmu.2018.01240/full

Citation 08

https://www.sciencedirect.com/science/article/pii/S0168365917309112

Citation 09

https://link.springer.com/article/10.1186/s10194-021-01306-7

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459684/

Citation 11

https://link.springer.com/article/10.1186/s12935-021-02387-9

Citation 12

https://biosignaling.biomedcentral.com/articles/10.1186/s12964-022-00879-3

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids.