Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit

Specifications

Catalog number

E-EL-H1603-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

VEGFR-2; KDR

UniProt

P35968

Cross Reactivity

This kit recognizes Human VEGFR-2/KDR in samples. No significant cross-reactivity or interference between Human VEGFR-2/KDR and analogues was observed

Target

VEGFR2; CD309; FLK1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Microbiology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGFR-2/KDR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGFR-2/KDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGFR-2/KDR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGFR-2/KDR. You can calculate the concentration of Human VEGFR-2/KDR in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1878535221002021

Citation 02

https://www.sciencedirect.com/science/article/pii/S0045206821007355

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.1c05007

Citation 04

https://www.sciencedirect.com/science/article/pii/S1567576922004155

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666787/

Citation 06

https://www.sciencedirect.com/science/article/pii/S0378874120310795

Citation 07

https://portlandpress.com/bioscirep/article/40/10/BSR20202130/226577

Citation 08

https://www.sciencedirect.com/science/article/pii/S0022286020312321

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761852/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human VEGFR-2/KDR concentrations in serum, plasma and other biological fluids.