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Product details

Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit

Catalog number E-EL-H1661-01

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Price for E-EL-H1661-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-H1661-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
UCP-1
UniProt
P25874
Cross Reactivity
This kit recognizes Human UCP-1 in samples. No significant cross-reactivity or interference between Human UCP-1 and analogues was observed
Target
SLC25A7; Thermogenin
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Metabolism; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human UCP-1. You can calculate the concentration of Human UCP-1 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.1 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0022395622006008
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human UCP-1 concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit?

The catalog number for Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit is E-EL-H1661.

What sizes are available for Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit?

Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit come in?

Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit is available in 3 different pack sizes.

Is Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit in stock?

Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit?

Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit?

Estimated delivery time for Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit?

The safety data sheet for Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit is available here: https://789.bio/eb/fnXTWT.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

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