Human GHRL (Ghrelin) ELISA Kit

Specifications

Catalog number

E-EL-H1919-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

GHRL

UniProt

Q9UBU3

Cross Reactivity

This kit recognizes Human GHRL in samples.No significant cross-reactivity or interference between Human GHRL and analogues was observed

Target

GHRL; MTLRP; Ghrelin

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human GHRL. During the reaction, Human GHRL in the sample or standard competes with a fixed amount of Human GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.frontiersin.org/articles/10.3389/fnut.2022.973291/full

Citation 02

https://link.springer.com/article/10.1007/s00394-021-02678-x

Citation 03

https://www.sciencedirect.com/science/article/pii/S1063458417310270

Citation 04

https://www.mdpi.com/1095382

Citation 05

https://www.sciencedirect.com/science/article/pii/S1043466621001617

Citation 06

https://www.sciencedirect.com/science/article/pii/S0195666321007625

Citation 07

https://www.sciencedirect.com/science/article/pii/S0143417921000500

Citation 08

https://www.mdpi.com/1660-4601/16/22/4464

Citation 09

https://www.sciencedirect.com/science/article/pii/S0165178117309642

Citation 10

https://www.hindawi.com/journals/bmri/2018/9784083/abs/

Citation 11

https://www.tandfonline.com/doi/abs/10.1080/08039488.2020.1772363

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8802860/

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human GHRL concentrations in serum, plasma and other biological fluids.