Human BMG/β2-MG (Beta-2-Microglobulin) ELISA Kit

Specifications

Catalog number

E-EL-H2188-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

BMG; β2-MG

UniProt

P61769

Cross Reactivity

This kit recognizes Human BMG/β2-MG in samples.No significant cross-reactivity or interference between Human BMG/β2-MG and analogues was observed

Target

B2MG; B2M

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human BMG/β2-MG. During the reaction, Human BMG/β2-MG in the sample or standard competes with a fixed amount of Human BMG/β2-MG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BMG/β2-MG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human BMG/β2-MG in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/1422-0067/17/5/631

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human BMG/β2-MG concentrations in serum, plasma and other biological fluids.