Human GLP-2 (Glucagon Like Peptide 2) ELISA Kit

Specifications

Catalog number

E-EL-H2238-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

GLP-2

UniProt

P01275

Cross Reactivity

This kit recognizes Human GLP-2 in samples.No significant cross-reactivity or interference between Human GLP-2 and analogues was observed

Target

GLP2

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human GLP-2. During the reaction, Human GLP-2 in the sample or standard competes with a fixed amount of Human GLP-2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human GLP-2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human GLP-2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2072-6643/12/6/1736

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human GLP-2 concentrations in serum, plasma and other biological fluids.