Human Ang1-7 (Angiotensin 1-7) ELISA Kit

Specifications

Catalog number

E-EL-H5518-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Ang1-7

UniProt

P01019

Cross Reactivity

This kit recognizes Human Ang1-7 in samples.No significant cross-reactivity or interference between Human Ang1-7 and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang1-7. During the reaction, Human Ang1-7 in the sample or standard competes with a fixed amount of Human Ang1-7 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang1-7. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang1-7 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://cardiab.biomedcentral.com/articles/10.1186/s12933-022-01573-x

Citation 02

https://link.springer.com/article/10.1186/s12575-022-00177-9

Citation 03

https://static.frontiersin.org/articles/10.3389/fendo.2022.912064/full

Citation 04

https://www.mdpi.com/1422-0067/21/11/3861

Citation 05

https://www.sciencedirect.com/science/article/pii/S0939475322000461

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332217342154

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human Ang1-7 concentrations in serum, plasma and other biological fluids.