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Product details

Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit

Catalog number E-EL-H5558-01

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Price for E-EL-H5558-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-H5558-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
SRANKL
UniProt
O14788
Cross Reactivity
This kit recognizes Human sRANKL in samples. No significant cross-reactivity or interference between Human sRANKL and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sRANKL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sRANKL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sRANKL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sRANKL. You can calculate the concentration of Human sRANKL in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8335545/
Citation 02
https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11990
Citation 03
https://www.frontiersin.org/articles/10.3389/fphar.2020.609119/full
Citation 04
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118287/
Citation 05
https://europepmc.org/article/pmc/pmc9908480?javascript_support=no
Citation 06
https://www.hindawi.com/journals/jir/2019/6195258/abs/
Citation 07
https://www.sciencedirect.com/science/article/pii/S1043466618304095
Citation 08
https://www.sciencedirect.com/science/article/pii/S0099239919306995
Citation 09
https://journals.sagepub.com/doi/abs/10.1177/1534734619859730
Citation 10
https://journals.lww.com/jpho-online/Fulltext/9900/OPG_RANK_RANKL_Axis_in_Egyptian_Children_With.151.aspx
Citation 11
https://bmcmusculoskeletdisord.biomedcentral.com/articles/10.1186/s12891-018-2356-4
Citation 12
https://www.jstage.jst.go.jp/article/josnusd/59/3/59_16-0677/_article/-char/ja/
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human sRANKL concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit?

The catalog number for Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit is E-EL-H5558.

What sizes are available for Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit?

Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit come in?

Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit is available in 3 different pack sizes.

Is Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit in stock?

Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit?

Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit?

Estimated delivery time for Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit?

The safety data sheet for Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit is available here: https://789.bio/eb/GWP4qT.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

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