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Product details

Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit

Catalog number E-EL-H5813-01

Discontinued

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Size / count 24 Tests

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Specifications

Catalog number
E-EL-H5813-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
Yes
CAS Number
7732-18-5
Abbreviation
RANκL
Synonyms
TNFSF11; TRANCE; RANKL; CD254; ODF; OPGL; OPTB2; hRANKL2; sOdf;
UniProt
O14788
Species Reactivity
Human
Cross Reactivity
This kit recognizes Human RANκL in samples.No significant cross-reactivity or interference between Human RANκL and analogues was observed
Target
Human
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Immunology
Detection Method
Colormetric
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RANκL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RANκL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RANκL. You can calculate the concentration of Human RANκL in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,12 months
Applications Notes
This ELISA kit applies to the in vitro quantitative determination of Human RANκL concentrations in serum, plasma and other biological fluids.
Citation 01
https://europepmc.org/article/med/34722902?javascript_support=no

Frequently Asked Questions

What is the catalog number for Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit?

The catalog number for Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit is E-EL-H5813.

Is Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit in stock?

Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit?

Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit?

Estimated delivery time for Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit?

The safety data sheet for Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit is available here: https://789.bio/eb/WPi5m5.

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

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