Human LEP (Leptin) ELISA Kit

Specifications

Catalog number

E-EL-H6017-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

LEP

UniProt

P41159

Cross Reactivity

This kit recognizes Human LEP in samples.No significant cross-reactivity or interference between Human LEP and analogues was observed

Target

LEPD; OBS; Obesity Homolog

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LEP. You can calculate the concentration of Human LEP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0956566319303367

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349898/

Citation 03

https://www.sciencedirect.com/science/article/pii/S001393511930862X

Citation 04

https://jlb.onlinelibrary.wiley.com/doi/abs/10.1002/JLB.5HI1020-704R

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890605/

Citation 06

https://www.mdpi.com/1660-4601/19/22/15195

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/jop.12819

Citation 08

https://www.nature.com/articles/s41598-021-87583-3

Citation 09

https://www.nature.com/articles/s41598-021-87583-3

Citation 10

https://link.springer.com/article/10.1007/s40620-021-00972-9

Citation 11

https://www.tandfonline.com/doi/abs/10.1080/01480545.2022.2058010

Citation 12

https://www.tandfonline.com/doi/abs/10.1080/09546634.2021.1901845

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human LEP concentrations in serum, plasma and other biological fluids.