Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit

Specifications

Catalog number

E-EL-H6042-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

BFGF; FGF2

UniProt

P09038

Cross Reactivity

This kit recognizes Human bFGF/FGF2 in samples.No significant cross-reactivity or interference between Human bFGF/FGF2 and analogues was observed

Target

FGF-2; B-FGF; BFGF; FGFB; HBGF-2; prostatropin; heparin-binding growth factor 2; FGF-β

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Developmental biology; Neuroscience; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human bFGF/FGF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human bFGF/FGF2. You can calculate the concentration of Human bFGF/FGF2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01461-2

Citation 02

https://pubs.acs.org/doi/abs/10.1021/acsami.2c13892

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13316

Citation 04

https://www.sciencedirect.com/science/article/pii/S075333222300077X

Citation 05

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-02739-w

Citation 06

https://www.mdpi.com/article/10.3390/ijms23137070

Citation 07

https://pubs.rsc.org/en/content/articlelanding/2020/bm/d0bm00800a

Citation 08

https://www.karger.com/Article/Abstract/490139

Citation 09

https://www.mdpi.com/1507072

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.28844

Citation 11

https://www.sciencedirect.com/science/article/pii/S0753332218384737

Citation 12

https://www.mdpi.com/1420-3049/24/18/3316

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human bFGF/FGF2 concentrations in serum, plasma and other biological fluids.