Human MMP-1 (Matrix Metalloproteinase 1) ELISA Kit

Specifications

Catalog number

E-EL-H6073-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

MMP-1

UniProt

P03956

Cross Reactivity

This kit recognizes Human MMP-1 in samples.No significant cross-reactivity or interference between Human MMP-1 and analogues was observed

Target

MMP1; CLG; CLGN; Interstitial Collagenase; Fibroblast Collagenase

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-1. You can calculate the concentration of Human MMP-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41467-021-23789-3

Citation 02

https://www.mdpi.com/2076-3921/11/10/1900

Citation 03

https://www.mdpi.com/1400396

Citation 04

https://www.sciencedirect.com/science/article/pii/S1011134422000951

Citation 05

https://europepmc.org/article/pmc/pmc9478898?javascript_support=no

Citation 06

https://www.sciencedirect.com/science/article/pii/S0926669022006471

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342544/

Citation 08

https://www.mdpi.com/1349972

Citation 09

https://www.sciencedirect.com/science/article/pii/S0009279721003756

Citation 10

https://www.mdpi.com/1305798

Citation 11

https://www.sciencedirect.com/science/article/pii/S0378874121010217

Citation 12

https://www.sciencedirect.com/science/article/pii/S1529943022000353

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human MMP-1 concentrations in serum, plasma and other biological fluids.