Human HBsAg (Hepatitis B surface antigen) ELISA Kit

Specifications

Catalog number

E-EL-H6080-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

HBsAg

UniProt

P30019

Cross Reactivity

This kit recognizes Human HBsAg in samples.No significant cross-reactivity or interference between Human HBsAg and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Microbiology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HBsAg. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HBsAg and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HBsAg, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HBsAg. You can calculate the concentration of Human HBsAg in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.28620

Citation 02

https://www.sciencedirect.com/science/article/pii/S0003267016306043

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/jvh.13809

Citation 04

https://www.spandidos-publications.com/ijmm/41/4/2169

Citation 05

https://www.sciencedirect.com/science/article/pii/S0027968417300998

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human HBsAg concentrations in serum, plasma and other biological fluids.