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Product details

Human IgE (Immunoglobulin E) ELISA Kit

Catalog number E-EL-H6104-01

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Price for E-EL-H6104-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-H6104-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
IgE
UniProt
P01854
Cross Reactivity
This kit recognizes Human IgE in samples.No significant cross-reactivity or interference between Human IgE and analogues was observed
Target
IgE
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgE. You can calculate the concentration of Human IgE in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
3.13-200 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
1.88 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346992/
Citation 02
https://www.sciencedirect.com/science/article/pii/S1876034121003993
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human IgE concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Human IgE (Immunoglobulin E) ELISA Kit?

The catalog number for Human IgE (Immunoglobulin E) ELISA Kit is E-EL-H6104.

What sizes are available for Human IgE (Immunoglobulin E) ELISA Kit?

Human IgE (Immunoglobulin E) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Human IgE (Immunoglobulin E) ELISA Kit come in?

Human IgE (Immunoglobulin E) ELISA Kit is available in 3 different pack sizes.

Is Human IgE (Immunoglobulin E) ELISA Kit in stock?

Human IgE (Immunoglobulin E) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Human IgE (Immunoglobulin E) ELISA Kit?

Human IgE (Immunoglobulin E) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Human IgE (Immunoglobulin E) ELISA Kit?

Estimated delivery time for Human IgE (Immunoglobulin E) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Human IgE (Immunoglobulin E) ELISA Kit?

The safety data sheet for Human IgE (Immunoglobulin E) ELISA Kit is available here: https://789.bio/eb/8uTWrP.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

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