Human BNP (Brain Natriuretic Peptide) ELISA Kit

Specifications

Catalog number

E-EL-H6166-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

BNP

UniProt

P16860

Cross Reactivity

This kit recognizes Human BNP in samples.No significant cross-reactivity or interference between Human BNP and analogues was observed

Target

GH receptor; GH-binding protein; GHBP; Serum binding protein

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BNP. You can calculate the concentration of Human BNP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

125-8000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s12276-021-00720-w

Citation 02

https://www.nature.com/articles/s41419-021-03777-7

Citation 03

https://www.sciencedirect.com/science/article/pii/S2001037020304761

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332221003164

Citation 05

https://www.nature.com/articles/srep21357

Citation 06

https://www.cambridge.org/core/journals/cardiology-in-the-young/article/evaluation-of-the-relationship-between-ventricular-function-and-serum-growth-differentiation-factor15-levels-in-patients-with-operated-tetralogy-of-fallot/261A6BBF5E31A0B9961EEC9DB43716E6

Citation 07

https://www.spandidos-publications.com/mmr/16/5/7766?text=fulltext

Citation 08

https://clinicalhypertension.biomedcentral.com/articles/10.1186/s40885-022-00221-6

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929926/

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1002/adbi.202200130

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human BNP concentrations in serum, plasma and other biological fluids.