Mouse BNP (Brain Natriuretic Peptide) ELISA Kit

Specifications

Catalog number

E-EL-M0204-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

BNP

UniProt

P40753

Cross Reactivity

This kit recognizes Mouse BNP in samples.No significant cross-reactivity or interference between Mouse BNP and analogues was observed

Target

NPPB; GC-B; B-Type Natriuretic Peptide; Natriuretic Peptide Precursor B

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Neuroscience

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse BNP. During the reaction, Mouse BNP in the sample or standard competes with a fixed amount of Mouse BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2211383522004798

Citation 02

https://www.nature.com/articles/s41401-022-01025-y

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332221009173

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332221003164

Citation 05

https://static.frontiersin.org/articles/10.3389/fcvm.2022.903316/full

Citation 06

https://www.ahajournals.org/doi/abs/10.1161/JAHA.120.019961

Citation 07

https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-021-03022-x

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.17160

Citation 09

https://www.mdpi.com/2038768

Citation 10

https://www.mdpi.com/1867006

Citation 11

https://europepmc.org/article/med/25056115?javascript_support=no

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1111/1440-1681.13699

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse BNP concentrations in serum, plasma and other biological fluids.