Mouse PINP (Procollagen I N-Terminal Propeptide) ELISA Kit

Specifications

Catalog number

E-EL-M0233-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

PINP

UniProt

P02452

Cross Reactivity

This kit recognizes Mouse PINP in samples. No significant cross-reactivity or interference between Mouse PINP and analogues was observed

Target

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Signal Transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PINP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PINP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PINP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PINP. You can calculate the concentration of Mouse PINP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

93.75-6000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

56.25 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41418-020-00731-6

Citation 02

https://www.nature.com/articles/s41467-021-27629-2

Citation 03

https://pubs.acs.org/doi/abs/10.1021/jacs.0c07309

Citation 04

https://www.embopress.org/doi/abs/10.15252/emmm.202115373

Citation 05

https://www.sciencedirect.com/science/article/pii/S2213231721003402

Citation 06

https://www.sciencedirect.com/science/article/pii/S1525001620301842

Citation 07

https://www.sciencedirect.com/science/article/pii/S2211124720300516

Citation 08

https://www.sciencedirect.com/science/article/pii/S0891584922005147

Citation 09

https://www.sciencedirect.com/science/article/pii/S0891584922009893

Citation 10

https://www.sciencedirect.com/science/article/pii/S0753332222010113

Citation 11

https://pubs.rsc.org/en/content/articlelanding/2021/bm/d1bm00563d

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8741853/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse PINP concentrations in serum, plasma and other biological fluids.