Mouse COL1 (Collagen Type I) ELISA Kit

Specifications

Catalog number

E-EL-M0325-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

COL1

UniProt

P11087 (COL1A1)

Cross Reactivity

This kit recognizes Mouse COL1 in samples. No significant cross-reactivity or interference between Mouse COL1 and analogues was observed

Target

COL1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Signal Transduction; Stem Cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse COL1. You can calculate the concentration of Mouse COL1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.frontiersin.org/articles/10.3389/fphar.2018.00628/full

Citation 02

https://www.frontiersin.org/articles/10.3389/fimmu.2020.01598/full?report=reader

Citation 03

https://www.sciencedirect.com/science/article/pii/S0141813020331561

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16398

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695362/

Citation 06

https://link.springer.com/article/10.1007/s13770-021-00425-1

Citation 07

https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-021-04952-7

Citation 08

https://www.worldscientific.com/doi/abs/10.1142/S0192415X19500575

Citation 09

https://www.hindawi.com/journals/bmri/2023/9950037/

Citation 10

https://www.sciencedirect.com/science/article/pii/S002013832200612X

Citation 11

https://www.spandidos-publications.com/10.3892/ol.2018.8989

Citation 12

https://www.sciencedirect.com/science/article/pii/S0975947617300074

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse COL1 concentrations in serum, plasma and other biological fluids.