Mouse CRH (Corticotropin Releasing Hormone) ELISA Kit

Specifications

Catalog number

E-EL-M0351-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

CRH

UniProt

Q8CIT0

Cross Reactivity

This kit recognizes Mouse CRH in samples.No significant cross-reactivity or interference between Mouse CRH and analogues was observed

Target

CRF; Corticoliberin

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse CRH. During the reaction, Mouse CRH in the sample or standard competes with a fixed amount of Mouse CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse CRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse CRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/cjoc.202000652

Citation 02

https://www.sciencedirect.com/science/article/pii/S0944711320302671

Citation 03

https://www.frontiersin.org/articles/10.3389/fcimb.2017.00489/full

Citation 04

https://www.nature.com/articles/s41598-021-82604-7

Citation 05

https://pubmed.ncbi.nlm.nih.gov/30683828/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse CRH concentrations in serum, plasma and other biological fluids.