Mouse DAO (Diamine Oxidase) ELISA Kit

Specifications

Catalog number

E-EL-M0412-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

DAO

UniProt

Q8JZQ5

Cross Reactivity

This kit recognizes Mouse DAO in samples. No significant cross-reactivity or interference between Mouse DAO and analogues was observed

Target

AOC1; ABP1; Amiloride Binding Protein 1; Amine Oxidase (Copper-Containing)

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DAO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse DAO. You can calculate the concentration of Mouse DAO in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.91-250 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

2.35 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1043661820315401

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332220304546

Citation 03

https://www.sciencedirect.com/science/article/pii/S1567576922001631

Citation 04

https://www.frontiersin.org/articles/10.3389/fphar.2020.00918/full

Citation 05

https://www.sciencedirect.com/science/article/pii/S0899900721004469

Citation 06

https://www.mdpi.com/2072-6651/12/12/794

Citation 07

https://anatomypubs.onlinelibrary.wiley.com/doi/abs/10.1002/ar.25065

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse DAO concentrations in serum, plasma and other biological fluids.