Mouse GHRL (Ghrelin) ELISA Kit

Specifications

Catalog number

E-EL-M0551-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

GHRL

UniProt

Q9EQX0

Cross Reactivity

This kit recognizes Mouse GHRL in samples.No significant cross-reactivity or interference between Mouse GHRL and analogues was observed

Target

GHRL; MTLRP; Ghrelin

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse GHRL. During the reaction, Mouse GHRL in the sample or standard competes with a fixed amount of Mouse GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0092867422015379

Citation 02

https://www.tandfonline.com/doi/abs/10.1080/19490976.2021.1987782

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202000490

Citation 04

https://www.tandfonline.com/doi/abs/10.1080/13813455.2021.1894178

Citation 05

https://www.sciencedirect.com/science/article/pii/S1756464621002309

Citation 06

https://www.sciencedirect.com/science/article/pii/S0041008X20302507

Citation 07

https://www.sciencedirect.com/science/article/pii/S0041008X19302510

Citation 08

https://www.sciencedirect.com/science/article/pii/S0024320520301909

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse GHRL concentrations in serum, plasma and other biological fluids.