Mouse GFAP (glial fibrillary acidic protein) ELISA Kit

Specifications

Catalog number

E-EL-M0554-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

7732-18-5

Abbreviation

GFAP

UniProt

P03995

Species Reactivity

Mouse

Cross Reactivity

This kit recognizes Mouse GFAP in samples. No significant cross-reactivity or interference between Mouse GFAP and analogues was observed

Target

Mouse

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience; Signal transduction; Stem cells; Tags and Cell Markers

Detection Method

Colormetric

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GFAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GFAP. You can calculate the concentration of Mouse GFAP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C/-20°C,12 months

Applications Notes

This ELISA kit applies to the in vitro quantitative determination of Mouse GFAP concentrations in serum, plasma and other biological fluids.