Mouse IgG (Immunoglobulin G) ELISA Kit

Specifications

Catalog number

E-EL-M0692-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IgG

UniProt

P01863

Cross Reactivity

This kit recognizes Mouse IgG in samples. No significant cross-reactivity or interference between Mouse IgG and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG. You can calculate the concentration of Mouse IgG in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8812323/

Citation 02

https://link.springer.com/article/10.1186/s12979-018-0142-7

Citation 03

https://www.nature.com/articles/s42003-023-04454-5

Citation 04

https://www.sciencedirect.com/science/article/pii/S2589004222006757

Citation 05

https://www.nature.com/articles/srep34079

Citation 06

https://academic.oup.com/jn/advance-article-abstract/doi/10.1093/jn/nxab328/6391405

Citation 07

https://www.sciencedirect.com/science/article/pii/S1818087617309704

Citation 08

https://pubs.rsc.org/en/content/articlehtml/2020/fo/d0fo01841d

Citation 09

https://www.sciencedirect.com/science/article/pii/S1756464622000901

Citation 10

https://www.sciencedirect.com/science/article/pii/S1756464622001402

Citation 11

https://www.mdpi.com/1603134

Citation 12

https://www.mdpi.com/2072-6651/12/12/794

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IgG concentrations in serum, plasma and other biological fluids.