Mouse NT-proBNP (N-terminal pro-Brain Natriuretic Peptide) ELISA Kit

Specifications

Catalog number

E-EL-M0834-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

NT-proBNP

UniProt

P40753

Cross Reactivity

This kit recognizes Mouse NT-proBNP in samples. No significant cross-reactivity or interference between Mouse NT-proBNP and analogues was observed

Target

NT-Pro-BNP

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NT-proBNP. You can calculate the concentration of Mouse NT-proBNP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2211383522004798

Citation 02

https://www.frontiersin.org/articles/10.3389/fcvm.2022.864188/full

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332221003164

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332222006692

Citation 05

https://www.sciencedirect.com/science/article/pii/S0753332222005480

Citation 06

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202101030R

Citation 07

https://www.sciencedirect.com/science/article/pii/S0014299922002837

Citation 08

https://link.springer.com/article/10.1007/s12264-020-00544-0

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724347/

Citation 10

https://www.nature.com/articles/s41598-020-65255-y

Citation 11

https://www.mdpi.com/1422-0067/18/12/2664

Citation 12

https://www.hindawi.com/journals/cdtp/2022/9615674/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse NT-proBNP concentrations in serum, plasma and other biological fluids.