Mouse NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit

Specifications

Catalog number

E-EL-M0838-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

NFKB-p65

UniProt

Q04207

Cross Reactivity

This kit recognizes Mouse NFKB-p65 in samples. No significant cross-reactivity or interference between Mouse NFKB-p65 and analogues was observed

Target

NFKB3; p65; RelA

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Microbiology; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NFKB-p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NFKB-p65. You can calculate the concentration of Mouse NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo04205j

Citation 02

https://www.mdpi.com/1420-3049/27/15/4992

Citation 03

https://www.sciencedirect.com/science/article/pii/S0161813X21000322

Citation 04

https://www.sciencedirect.com/science/article/pii/S0041008X19302510

Citation 05

https://www.sciencedirect.com/science/article/pii/S0024320520301909

Citation 06

https://pubmed.ncbi.nlm.nih.gov/34374661/

Citation 07

https://www.spandidos-publications.com/mmr/17/5/6752?text=fulltext

Citation 08

https://www.spandidos-publications.com/ol/15/5/6352

Citation 09

https://www.sciencedirect.com/science/article/pii/S2213434422000111

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse NFKB-p65 concentrations in serum, plasma and other biological fluids.