Mouse OC/BGP (Osteocalcin) ELISA Kit

Specifications

Catalog number

E-EL-M0864-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

OC; BGP

UniProt

P86546

Cross Reactivity

This kit recognizes Mouse OC/BGP in samples. No significant cross-reactivity or interference between Mouse OC/BGP and analogues was observed

Target

BGLAP; OT; Bone Gamma-carboxyglutamate Protein; Bone Gla Protein

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse OC/BGP. You can calculate the concentration of Mouse OC/BGP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.39-25 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.23 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202004831

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202100808

Citation 03

https://www.nature.com/articles/s41467-022-29191-x

Citation 04

https://www.sciencedirect.com/science/article/pii/S1534580722006839

Citation 05

https://www.nature.com/articles/s41413-019-0056-9

Citation 06

https://www.tandfonline.com/doi/abs/10.1080/15548627.2020.1839286

Citation 07

https://www.sciencedirect.com/science/article/pii/S1525001620301842

Citation 08

https://www.nature.com/articles/cdd201672

Citation 09

https://www.sciencedirect.com/science/article/pii/S0891584922005147

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086346/

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019162/

Citation 12

https://www.sciencedirect.com/science/article/pii/S0753332222007703

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse OC/BGP concentrations in serum, plasma and other biological fluids.