Mouse PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Specifications

Catalog number

E-EL-M0959-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

PTGS2; COX-2

UniProt

Q05769

Cross Reactivity

This kit recognizes Mouse PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Mouse PTGS2/COX-2 and analogues was observed

Target

COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PTGS2/COX-2. You can calculate the concentration of Mouse PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.acs.org/doi/abs/10.1021/acsami.2c14448

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.9b01663

Citation 04

https://www.sciencedirect.com/science/article/pii/S0161589022001936

Citation 05

https://www.sciencedirect.com/science/article/pii/S1567576922001989

Citation 06

https://www.hindawi.com/journals/omcl/2019/5813985/abs/

Citation 07

https://www.sciencedirect.com/science/article/pii/S0968089620303527

Citation 08

https://pubs.acs.org/doi/abs/10.1021/acs.chemrestox.1c00317

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976907/

Citation 10

https://www.sciencedirect.com/science/article/pii/S2221169117310687

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.