Mouse TSH (Thyroid Stimulating Hormone) ELISA Kit

Specifications

Catalog number

E-EL-M1153-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

TSH

UniProt

P12656

Cross Reactivity

This kit recognizes Mouse TSH in samples. No significant cross-reactivity or interference between Mouse TSH and analogues was observed

Target

Thyrotropin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TSH. You can calculate the concentration of Mouse TSH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.25-80 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.75 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/smtd.201700269

Citation 02

https://www.sciencedirect.com/science/article/pii/S1043661823000415

Citation 03

https://www.mdpi.com/1422-0067/23/15/8244

Citation 04

https://www.sciencedirect.com/science/article/pii/S0147651319301538

Citation 05

https://www.hindawi.com/journals/omcl/2021/2482453/

Citation 06

https://www.liebertpub.com/doi/abs/10.1089/thy.2019.0557

Citation 07

https://www.hindawi.com/journals/jir/2022/7455494/

Citation 08

https://link.springer.com/content/pdf/10.1007/s11356-020-08210-8.pdf

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.13753

Citation 10

https://link.springer.com/article/10.1186/s12958-019-0536-1

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse TSH concentrations in serum, plasma and other biological fluids.