Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) ELISA Kit

Specifications

Catalog number

E-EL-M1203-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

TNNI3; cTn-I

UniProt

P48787

Cross Reactivity

This kit recognizes Mouse TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Mouse TNNI3/cTn-I and analogues was observed

Target

TNNC1; cTnI; CMD1FF; CMD2A; CMH7; RCM1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNNI3/cTn-I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNNI3/cTn-I. You can calculate the concentration of Mouse TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://cardiab.biomedcentral.com/articles/10.1186/s12933-022-01532-6

Citation 02

https://www.sciencedirect.com/science/article/pii/S0944711322004159

Citation 03

https://www.mdpi.com/2072-6694/14/12/2894

Citation 04

https://www.dovepress.com/protective-effect-of-crocin-on-immune-checkpoint-inhibitors-related-my-peer-reviewed-fulltext-article-JIR

Citation 05

https://link.springer.com/article/10.1186/s13287-021-02325-6

Citation 06

https://pubmed.ncbi.nlm.nih.gov/35083298/

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332221012403

Citation 08

https://www.sciencedirect.com/science/article/pii/S0753332222005510

Citation 09

https://link.springer.com/article/10.1186/s13020-021-00465-8

Citation 10

https://www.sciencedirect.com/science/article/pii/S0944711322003622

Citation 11

https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-021-03022-x

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7906200/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse TNNI3/cTn-I concentrations in serum, plasma and other biological fluids.