Mouse Ang-II (Angiotensin II) ELISA Kit

Specifications

Catalog number

E-EL-M2612-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Ang-II

UniProt

P11859

Cross Reactivity

This kit recognizes Mouse Ang-II in samples.No significant cross-reactivity or interference between Mouse Ang-II and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Ang-II. During the reaction, Mouse Ang-II in the sample or standard competes with a fixed amount of Mouse Ang-II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse Ang-II. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Ang-II in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ahajournals.org/doi/abs/10.1161/HYPERTENSIONAHA.119.12941

Citation 02

https://elifesciences.org/articles/42628

Citation 03

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202101030R

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.17160

Citation 05

https://iovs.arvojournals.org/article.aspx?articleid=2783641

Citation 06

https://www.sciencedirect.com/science/article/pii/S0271531720304450

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007412/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse Ang-II concentrations in serum, plasma and other biological fluids.