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Product details

Mouse INS (Insulin) ELISA Kit

Catalog number E-EL-M2614-01

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Price for E-EL-M2614-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-M2614-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
INS
UniProt
P01325, P01326
Cross Reactivity
This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed
Target
IDDM2; ILPR; IRDN; MODY10
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Cell culture supernatant and tissue homogenate
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.nature.com/articles/s41419-021-04160-2
Citation 02
https://www.sciencedirect.com/science/article/pii/S0963996922012650
Citation 03
https://www.sciencedirect.com/science/article/pii/S1043661818301683
Citation 04
https://pubs.acs.org/doi/abs/10.1021/acs.jafc.0c01467
Citation 05
https://www.sciencedirect.com/science/article/pii/S0014482719303076
Citation 06
https://link.springer.com/article/10.1007/s11356-018-1337-3
Citation 07
https://www.spandidos-publications.com/10.3892/mmr.2022.12686
Citation 08
https://www.hindawi.com/journals/ecam/2021/5514175/
Citation 09
https://journals.sagepub.com/doi/abs/10.1177/1535370221997780
Citation 10
https://link.springer.com/article/10.1007/s00795-019-00216-7
Citation 11
https://link.springer.com/article/10.1007/s40200-022-01135-8
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in cell culture supernatant and tissue homogenate.

Frequently Asked Questions

What is the catalog number for Mouse INS (Insulin) ELISA Kit?

The catalog number for Mouse INS (Insulin) ELISA Kit is E-EL-M2614.

What sizes are available for Mouse INS (Insulin) ELISA Kit?

Mouse INS (Insulin) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Mouse INS (Insulin) ELISA Kit come in?

Mouse INS (Insulin) ELISA Kit is available in 3 different pack sizes.

Is Mouse INS (Insulin) ELISA Kit in stock?

Mouse INS (Insulin) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Mouse INS (Insulin) ELISA Kit?

Mouse INS (Insulin) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Mouse INS (Insulin) ELISA Kit?

Estimated delivery time for Mouse INS (Insulin) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Mouse INS (Insulin) ELISA Kit?

The safety data sheet for Mouse INS (Insulin) ELISA Kit is available here: https://789.bio/eb/HyDK0O.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

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