Mouse LEP (Leptin) ELISA Kit

Specifications

Catalog number

E-EL-M3008-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

LEP

UniProt

P41160

Cross Reactivity

This kit recognizes Mouse LEP in samples.No significant cross-reactivity or interference between Mouse LEP and analogues was observed

Target

LEPD; OB; OBS; Obesity Homolog

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://academic.oup.com/biomedgerontology/article-abstract/76/3/393/5813438

Citation 02

https://www.sciencedirect.com/science/article/pii/S0891584918314394

Citation 03

https://www.sciencedirect.com/science/article/pii/S0269749120363867

Citation 04

https://www.mdpi.com/1042904

Citation 05

https://www.sciencedirect.com/science/article/pii/S0045653519329017

Citation 06

https://www.mdpi.com/1422-0067/21/14/5121

Citation 07

https://www.mdpi.com/2218-273X/10/10/1454

Citation 08

https://www.tandfonline.com/doi/abs/10.1080/13813455.2021.1894178

Citation 09

https://pubs.rsc.org/en/content/articlelanding/2020/fo/d0fo01405b

Citation 10

https://www.sciencedirect.com/science/article/pii/S001429992030296X

Citation 11

https://www.sciencedirect.com/science/article/pii/S1756464619300854

Citation 12

https://www.sciencedirect.com/science/article/pii/S0041008X20302507

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse LEP concentrations in serum, plasma and other biological fluids.