Mouse Aβ1-42 (Amyloid Beta 1-42) ELISA Kit

Specifications

Catalog number

E-EL-M3010-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Aβ1-42

UniProt

P12023

Cross Reactivity

This kit recognizes Mouse Aβ1-42 in samples.No significant cross-reactivity or interference between Mouse Aβ1-42 and analogues was observed

Target

Aβ42; Amyloid Beta 42

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Aβ1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Aβ1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Aβ1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Aβ1-42. You can calculate the concentration of Mouse Aβ1-42 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.13-200 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.tandfonline.com/doi/abs/10.1080/15548627.2019.1596488

Citation 02

https://www.sciencedirect.com/science/article/pii/S1043661822005412

Citation 03

https://europepmc.org/articles/pmc9530393/bin/datasheet_1.pdf?javascript_support=no

Citation 04

https://www.sciencedirect.com/science/article/pii/S0141813022015227

Citation 05

https://www.sciencedirect.com/science/article/pii/S0048969721038043

Citation 06

https://link.springer.com/article/10.1007/s13311-022-01204-z

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/cns.14102

Citation 08

https://www.sciencedirect.com/science/article/pii/S0141813022005499

Citation 09

https://www.mdpi.com/2072-6643/14/15/3179

Citation 10

https://www.mdpi.com/1883524

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7619

Citation 12

https://www.sciencedirect.com/science/article/pii/S0147651321004772

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse Aβ1-42 concentrations in serum, plasma and other biological fluids.