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Product details

Mouse IgE (Immunoglobulin E) ELISA Kit

Catalog number E-EL-M3034-01

Discontinued

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Size / count 24 Tests

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Specifications

Catalog number
E-EL-M3034-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
Yes
CAS Number
7732-18-5
Abbreviation
IgE
Synonyms
IgE
UniProt
F6W6U3
Species Reactivity
Mouse
Cross Reactivity
This kit recognizes Mouse IgE in samples.No significant cross-reactivity or interference between Mouse IgE and analogues was observed
Target
Mouse
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Immunology
Detection Method
Colormetric
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgE. You can calculate the concentration of Mouse IgE in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
125-8000 ng/mL
Precision
Both intra-CV and inter-CV are < 10%
Recovery
80%-120%
Sensitivity
75 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,12 months
Applications Notes
This ELISA kit applies to the in vitro quantitative determination of Mouse IgE concentrations in serum, plasma and other biological fluids.
Citation 01
https://www.sciencedirect.com/science/article/pii/S0048969722041730

Frequently Asked Questions

What is the catalog number for Mouse IgE (Immunoglobulin E) ELISA Kit?

The catalog number for Mouse IgE (Immunoglobulin E) ELISA Kit is E-EL-M3034.

Is Mouse IgE (Immunoglobulin E) ELISA Kit in stock?

Mouse IgE (Immunoglobulin E) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Mouse IgE (Immunoglobulin E) ELISA Kit?

Mouse IgE (Immunoglobulin E) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Mouse IgE (Immunoglobulin E) ELISA Kit?

Estimated delivery time for Mouse IgE (Immunoglobulin E) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Mouse IgE (Immunoglobulin E) ELISA Kit?

The safety data sheet for Mouse IgE (Immunoglobulin E) ELISA Kit is available here: https://789.bio/eb/SiTu5S.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

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