Mouse ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit

Specifications

Catalog number

E-EL-M3037-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ICAM-1; CD54

UniProt

P13597

Cross Reactivity

This kit recognizes Mouse ICAM-1/CD54 in samples.No significant cross-reactivity or interference between Mouse ICAM-1/CD54 and analogues was observed

Target

ICAM1; BB2; CD54; P3.58; intercellular adhesion molecule 1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ICAM-1/CD54. You can calculate the concentration of Mouse ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.37-1000 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.82 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/

Citation 02

https://www.mdpi.com/2072-6694/12/3/623

Citation 03

https://www.sciencedirect.com/science/article/pii/S0925443917300534

Citation 04

https://www.sciencedirect.com/science/article/pii/S0891584919308792

Citation 05

https://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.8b00781

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332220309495

Citation 07

https://www.nature.com/articles/s41374-019-0320-z

Citation 08

https://link.springer.com/article/10.1007/s12012-021-09697-4

Citation 09

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185688

Citation 10

https://www.sciencedirect.com/science/article/pii/S0895398819300455

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse ICAM-1/CD54 concentrations in serum, plasma and other biological fluids.