Rat BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit

Specifications

Catalog number

E-EL-R0002-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

BMP-2

UniProt

P49001

Cross Reactivity

This kit recognizes Rat BMP-2 in samples. No significant cross-reactivity or interference between Rat BMP-2 and analogues was observed

Target

BMP2A; BMP-2A; BDA2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BMP-2. You can calculate the concentration of Rat BMP-2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0142961222003258

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202201661

Citation 03

https://pubs.rsc.org/en/content/articlehtml/2020/bm/c9bm01352k

Citation 04

https://link.springer.com/article/10.1007/s42242-021-00172-1

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/clr.13602

Citation 06

https://www.sciencedirect.com/science/article/pii/S0045653515007572

Citation 07

https://www.ingentaconnect.com/content/ben/cpd/2019/00000025/00000027/art00008

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat BMP-2 concentrations in serum, plasma and other biological fluids.