Rat LH (Luteinizing Hormone) ELISA Kit

Specifications

Catalog number

E-EL-R0026-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

LH

UniProt

P01230

Cross Reactivity

This kit recognizes Rat LH in samples. No significant cross-reactivity or interference between Rat LH and analogues was observed

Target

Lutropin; Lutrophin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LH. You can calculate the concentration of Rat LH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 mIU/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 mIU/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41467-021-21308-y

Citation 02

https://www.sciencedirect.com/science/article/pii/S0269749122020589

Citation 03

https://www.sciencedirect.com/science/article/pii/S0144861722002089

Citation 04

https://www.sciencedirect.com/science/article/pii/S014765132200656X

Citation 05

https://www.hindawi.com/journals/omcl/2022/2113293/

Citation 06

https://www.sciencedirect.com/science/article/pii/S004896972100262X

Citation 07

https://www.mdpi.com/2076-3921/11/10/1879

Citation 08

https://biolres.biomedcentral.com/articles/10.1186/s40659-022-00401-6

Citation 09

https://www.sciencedirect.com/science/article/pii/S0024320522009833

Citation 10

https://www.mdpi.com/1422-0067/23/17/9759

Citation 11

https://www.hindawi.com/journals/omcl/2021/7382900/

Citation 12

https://www.sciencedirect.com/science/article/pii/S0753332222002591

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat LH concentrations in serum, plasma and other biological fluids.