Rat Bcl-2 (B-cell Lymphoma/Leukemia 2) ELISA Kit

Specifications

Catalog number

E-EL-R0096-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Bcl-2

UniProt

P49950

Cross Reactivity

This kit recognizes Rat Bcl-2 in samples. No significant cross-reactivity or interference between Rat Bcl-2 and analogues was observed

Target

Bcl2; PPP1R50

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Bcl-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Bcl-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Bcl-2. You can calculate the concentration of Rat Bcl-2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8722537/

Citation 02

https://www.sciencedirect.com/science/article/pii/S0024320522006397

Citation 03

https://www.sciencedirect.com/science/article/pii/S0048969720314820

Citation 04

https://www.sciencedirect.com/science/article/pii/S1567576923001649

Citation 05

https://www.sciencedirect.com/science/article/pii/S0278584621001275

Citation 06

https://www.sciencedirect.com/science/article/pii/S0024320521003738

Citation 07

https://www.mdpi.com/1950756

Citation 08

https://link.springer.com/article/10.1007/s11356-023-25917-6

Citation 09

https://www.mdpi.com/1742692

Citation 10

https://www.sciencedirect.com/science/article/pii/S0300483X21003188

Citation 11

https://www.frontiersin.org/articles/10.3389/fphar.2020.563750/full

Citation 12

https://link.springer.com/article/10.1007/s11064-022-03640-x

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat Bcl-2 concentrations in serum, plasma and other biological fluids.