Rat β-EP (Beta-Endorphin) ELISA Kit

Specifications

Catalog number

E-EL-R0105-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Β-EP

UniProt

P01194 (205~235aa)

Cross Reactivity

This kit recognizes Rat β-EP in samples.No significant cross-reactivity or interference between Rat β-EP and analogues was observed

Target

B-EP

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat β-EP. During the reaction, Rat β-EP in the sample or standard competes with a fixed amount of Rat β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/604796

Citation 02

https://www.mdpi.com/257188

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat β-EP concentrations in serum, plasma and other biological fluids.