Rat cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit

Specifications

Catalog number

E-EL-R0151-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

CTnT; TNNT2

UniProt

P50753

Cross Reactivity

This kit recognizes Rat cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Rat cTnT/TNNT2 and analogues was observed

Target

CMH2; CMPD2; LVNC6; RCM3; TnTC

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat cTnT/TNNT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat cTnT/TNNT2. You can calculate the concentration of Rat cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.hindawi.com/journals/omcl/2022/1090893/

Citation 02

https://www.hindawi.com/journals/omcl/2022/3712500/

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332222008265

Citation 04

https://www.sciencedirect.com/science/article/pii/S2001037020304761

Citation 05

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.201802707R

Citation 06

https://pubmed.ncbi.nlm.nih.gov/35957913/

Citation 07

https://www.sciencedirect.com/science/article/pii/S0378874122009540

Citation 08

https://www.mdpi.com/1127224

Citation 09

https://www.nature.com/articles/s41598-021-93234-4

Citation 10

https://www.sciencedirect.com/science/article/pii/S0271531722000549

Citation 11

https://www.frontiersin.org/articles/10.3389/fphar.2019.01493/full?report=reader

Citation 12

https://link.springer.com/article/10.1007/s12012-018-9478-7

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat cTnT/TNNT2 concentrations in serum, plasma and other biological fluids.