Rat CASP9 (Caspase 9) ELISA Kit

Specifications

Catalog number

E-EL-R0163-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

CASP9

UniProt

Q9JHK1

Cross Reactivity

This kit recognizes Rat CASP9 in samples. No significant cross-reactivity or interference between Rat CASP9 and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Metabolism

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP9. You can calculate the concentration of Rat CASP9 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0024320523001303

Citation 02

https://www.frontiersin.org/articles/10.3389/fphar.2020.610331/full

Citation 03

https://www.sciencedirect.com/science/article/pii/S1382668922002046

Citation 04

https://www.sciencedirect.com/science/article/pii/S0009279721002209

Citation 05

https://www.sciencedirect.com/science/article/pii/S1382668921002040

Citation 06

https://academic.oup.com/jpp/advance-article-abstract/doi/10.1093/jpp/rgac071/6760302

Citation 07

https://link.springer.com/article/10.1007/s12550-023-00474-6

Citation 08

https://www.sciencedirect.com/science/article/pii/S0300483X20301955

Citation 09

https://link.springer.com/article/10.1007/s11356-022-21075-3

Citation 10

https://www.sciencedirect.com/science/article/pii/S0041010121004621

Citation 11

https://www.sciencedirect.com/science/article/pii/S0041010122003397

Citation 12

https://academic.oup.com/toxres/article-abstract/10/2/345/6134433

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat CASP9 concentrations in serum, plasma and other biological fluids.