Rat D2D (D-Dimer) ELISA Kit

Specifications

Catalog number

E-EL-R0317-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

D2D

Cross Reactivity

This kit recognizes Rat D2D in samples.No significant cross-reactivity or interference between Rat D2D and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cardiovascular

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat D2D. During the reaction, Rat D2D in the sample or standard competes with a fixed amount of Rat D2D on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat D2D. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat D2D in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Plasma

Detection Range

78.13-5000 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0049384821004667

Citation 02

https://link.springer.com/article/10.1007/s00360-018-1186-x

Citation 03

https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-021-00708-4

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat D2D concentrations in plasma.