Rat AChE (Acetylcholinesterase) ELISA Kit

Specifications

Catalog number

E-EL-R0355-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

AChE

UniProt

P37136

Cross Reactivity

This kit recognizes Rat AChE in samples. No significant cross-reactivity or interference between Rat AChE and analogues was observed

Target

ACEE; ARACHE; N-ACHE; YT; acetylhydrolase

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AChE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AChE. You can calculate the concentration of Rat AChE in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.47 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0147651322009198

Citation 02

https://link.springer.com/article/10.1007/s12035-022-03074-0

Citation 03

https://europepmc.org/article/med/35991900?javascript_support=no

Citation 04

https://www.mdpi.com/436946

Citation 05

https://www.sciencedirect.com/science/article/pii/S0014299921008517

Citation 06

https://www.mdpi.com/article/10.3390/molecules27154907

Citation 07

https://www.sciencedirect.com/science/article/pii/S0147651318302975

Citation 08

https://link.springer.com/article/10.1007/s12640-022-00630-3

Citation 09

https://www.sciencedirect.com/science/article/pii/S0304394022000568

Citation 10

https://www.sciencedirect.com/science/article/pii/S0024320520316295

Citation 11

https://www.mdpi.com/2050368

Citation 12

https://www.sciencedirect.com/science/article/pii/S1319016420300645

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat AChE concentrations in serum, plasma and other biological fluids.